Production of monoclonal antibodies specific to the carboxyl terminal region of the 85 kDa subunit of phosphatidylinositol 3-kinase: Use of the antibodies in recognition of mutant p85
Major Publication 1. Sakda Daduang and Nunthawun Uawonggul, Chapter 92 Herbal Therapies of Snake and Insect Bites in Thailand, Encyclopedia of Botanicals Medicine in Clinical Practice (editor V.R. Preedy and R.R. Watson), (2008) p.814-822. 2. Nunthawun Uawonggul, Sompong Thammasirirak, Arunrat Chaveerach, Tarinee Arkaravichien, Wandee Bunyatratchata, Wipaporn Ruangjirachuporn, Pornpimol Jearranaiprepame, Takeshi Nakamura, Michiyuki Matsuda, Michimoto Kobayashi, Seisuke Hattori, Sakda Daduang (2007) Purification and Characterization of Heteroscorpine-1 (HS-1) toxin from Heterometrus laoticus Scorpion Venom. Toxicon 49(1) 19-29. (มีค่า Impact Factor 2.255, Year 2005) (เป็น corresponding author และสัดส่วนการมีส่วนร่วม 50%) 3. Nunthawun Uawonggul, Arunrat Chaveerach, Sompong Thammasirirak, Tarinee Arkaravichien, Chattong Chuachan and Sakda Daduang. (2006) Screening of plants acting against Heterometrus laoticus scorpion venom activity on fibroblast cell lysis. Journal of Ethnopharmacology 103(2); 201-207. (มีค่า Impact Factor 1.554, Year 2005) (เป็น corresponding author และสัดส่วนการมีส่วนร่วม 35%) 4. Sakda Daduang, Suporn Nuchadomrong and Panasun Dumrongwutinon (2005) Glycoprotein and biotin-coupled proteins as taxonomic markers of Cassia fistula. Botanica Lithuanica 11 (3); 135-140. (ไม่มีค่า Impact Factor) (เป็น corresponding author และสัดส่วนการมีส่วนร่วม 50%) 5. Sakda Daduang, Nison Sattayasai, Jintana Sattayasai, Pattara Tophrom, Achra Thammathaworn, Arunrat Chaveerach and Monruedee Konkchaiyaphum (2005). Screening of plants containing Naja naja siamensis cobra venom inhibitory activity using modified ELISA technique. Analytical Biochemistry 341(2); 316-325. (มีค่า Impact Factor 2.948, Year 2006) (เป็น corresponding author และสัดส่วนการมีส่วนร่วม 50%) 6. Sakda Daduang, Nunthawun Uawonggul, (2004) The Wonders and Mysteries of Asian Forest Scorpions. Sarakadee Magazine 19 (227) Jan 2004, 110-120. (in Thai) (สัดส่วนการมีส่วนร่วม 50%) 7. Nison Sattayasai, Jintana Sattayasai, Sakda Daduang, Thippayarat Chahomchuen, Somporn Ketkaew, Hathairat Puchongkavarin (2003) The nonmitochondrial carboxylase, which shows a relation to glutamate action, is last synthesized in retina of chick embryo. Journal of Neuroimmunology 141(1-2):104-111. (สัดส่วนการมีส่วนร่วม 15%) 8. Sakda Daduang, Nison Sattayasai, Jintana Sattayasai, Somporn Ketkaew, Thippayarat Chahomchuen and Rasamee Daengmark. (2000) Production of antibodies specific to chick retinal glutamate-sensitive protein. KKU Research Journal 5 (1) : 66-71. (สัดส่วนการมีส่วนร่วม 80%) 9. Daduang, S., Nagata, S., Kimura, K., and Fukui, Y.(1998) Density dependent elevation of phosphatidylinositol-3 kinase level in rat 3Y1 Cells. Biochim. Biophys. Acta. 1401(1), 113-120. 10. Daduang, S., Nagata, S., Matsuda, M., Yamori, T., Onodera, K. and Fukui, Y. (1995) Production of monoclonal antibodies specific to carboxyl terminal Region of 85 kDa subunit of phosphatidylinositol 3-kinase: use of the antibodies in recognition of the mutant p85s. Immunol. Cell Biol. 73(2), 134-9.
Major Presentation 1. Sakda Daduang Active Proteins In Scorpion, Wasp And Fire Ant Venom. The 2nd Biochemistry and Molecular Biology Conference on Biochemistry and Molecular Biology for Regional Sustainable Development. Faculty of Science, Khon Kaen University, Khon Kaen, Thailand, May 7-8, 2009. 2. Nunthawun Uawonggul, Prapenpuksiri Rungsa, Sophida Sukprasert, Paroonkorn Incamnoi, Sompong Thammasirirak, and Sakda Daduang. Phospholipase Activity From Thai King Cobra, Cobra, Scorpion, Wasp And Ant Venoms. The 2nd Biochemistry and Molecular Biology Conference on Biochemistry and Molecular Biology for Regional Sustainable Development. Faculty of Science, Khon Kaen University, Khon Kaen, Thailand, May 7-8, 2009. 3. Paroonkorn Incamnoi, Sompong Thammasirirak, Arunrat Chaveerach, Wandee Bunyatratchata, Nunthawun Uawonggul, and Sakda Daduang. Purification and Characterization of A Short' Imperatoxin-Like Peptide From Heterometrus Laoticus Venom. The 2nd Biochemistry and Molecular Biology Conference on Biochemistry and Molecular Biology for Regional Sustainable Development. Faculty of Science, Khon Kaen University, Khon Kaen, Thailand, May 7-8, 2009. 4. Prapenpuksiri Rungsa, Nunthawun Uawonggol, Sophida Sukprasert, Paroonkorn Incamnoi, Sompong Thammasirirak, and Sakda Daduang. Purification And Activity of Hyaluronidase from Thai Dead, Great Banded Wasps Venom (Vespa Tropica). The 2nd Biochemistry and Molecular Biology Conference on Biochemistry and Molecular Biology for Regional Sustainable Development. Faculty of Science, Khon Kaen University, Khon Kaen, Thailand, May 7-8, 2009. 5. Sophida Sukprasert, Theerawat Namonsai, Prapenpuksiri Rungsa, Nunthawun Uawonggul, Sompong Thammasirirak, Jureerut Daduang, and Sakda Daduang. Proteomic Study of The Thai Banded Wasp Venom, Vespa affinis. The 2nd Biochemistry and Molecular Biology Conference on Biochemistry and Molecular Biology for Regional Sustainable Development. Faculty of Science, Khon Kaen University, Khon Kaen, Thailand, May 7-8, 2009. 6. Siwaporn Lacheangkhong, Sompong Thammasirirak, Akhom Kesorn, and Sakda Daduang. Development of Strips for Human Blood Identification in Forensic Science Specimen. The 2nd Biochemistry and Molecular Biology Conference on Biochemistry and Molecular Biology for Regional Sustainable Development. Faculty of Science, Khon Kaen University, Khon Kaen, Thailand, May 7-8, 2009. 7. Natthaporn Kuendee, Ajjima Thongbo, Sompong Thammasirirak, and Sakda Daduang Development of Urease Strip for Mercury Detection. The 2nd Biochemistry and Molecular Biology Conference on Biochemistry and Molecular Biology for Regional Sustainable Development. Faculty of Science, Khon Kaen University, Khon Kaen, Thailand, May 7-8, 2009. 8. Prapenpuksiri Rungsa, Nunthawun Uawonggul, Sophida Sukprasert, Paroonkorn Incamnoi, Theerawat Namonsai, Sompong Thammasirirak and Sakda Daduang Main Protein Components in Thai Dead, Great Banded Hornet, Vespa Tropica, Venom The Third Annual Symposium of Protein Society of Thailand Frontiers in Protein Research Chulabhorn Research Institute Conference Center, Bangkok, Thailand, August 28-29, 2008. 9. Sophida Sukprasert, Nunthawun Uawonggul, Paroonkorn Incomnoi, Sompong Thammasirirak, Tasanee Jamjanya and Sakda Daduang, An Allergen from Thai Red Fire Ant, Solenopsis geminata : Characterization and Antibody Production 33rd FEBS Congress & 11th IUBMB Conference Biochemistry of Cell Regulation Megaron Athens International Conference Centre, Athens, Greece, June 28th to July 3rd, 2008 10. Sakda Daduang, Heteroscorpine (HS) toxin from Venom of Thai Giant Scorpion (Heterometrus sp.) with antibacterial activities. BIT Life Sciences 1st Annual Peptide and Protein Conference (PepCon-2008) From Concept to Market Shenzhen Convention & Exhibition Center, Shenzhen (เซินเจิ้น), People republic of China, 22-24 April 2008. 11. Sakda Daduang, Sompong Thammasirirak, Tassanee Jamjanya, Nunthawun Uawonggul, Sophida Sukprasert, Theerawat Namonsai, Amino acid sequence determination and investigation of activity of proteins and peptides in venom from local animals in the Northeastern part of Thailand, นักวิจัยรุ่นใหม่...พบ...เมธีวิจัยอาวุโส สกว. ครั้งที่ 7 ระหว่างวันที่ 11-13 ตุลาคม 2550 ณ โรงแรมแอมบาสเดอร์ ซิตี้ จอมเทียน เมืองพัทยา จ.ชลบุรี 12. Sakda Daduang, Nunthawun Uawonggul, Sompong Thammasirirak, Takeshi Nakamura, Michiyuki Matsuda, Bacterial Expression of Recombinant Heteroscorpine-1 (HS-1) Gene and Protein Purification, The Second Annual Symposium of Protein Society of Thailand Odysseys in Protein Research In Honor of Professor M.R. Jisnuson Svastis 60th Birthday. Chulabhorn Research Institute Conference Center, Bangkok, Thailand, September 20-21, 2007. 13. Sophida Sukprasert, Sompong Thammasirirak, Tasanee Jamjanya and Sakda Daduang การแยกบริสุทธิ์โปรตีนออกฤทธิ์ในน้ำพิษมดคันไฟไทย (Solenopsis geminata) Purification of an active protein in venom of Thai Red fire ant (Solenopsis geminata) The 32st Congress on Science and Technology of Thailand, 10-12 Oct 2006, Queen Sirikit National Convention Center, Bangkok Thailand 14. Nunthawun Uawonggul, Wipaporn Ruangjirachuporn, Tarinee Arkaravichien, Pornpimol Jearranaiprepame, Arunrat Chaveerach, Sompong Thammasirirak, Wandee Bunyatratchata and Sakda Daduang, Anti-acanthamoebic activity of Heteroscorpine-1 (HS-1) Neurotoxin from Heterometrus laoticus Thai Scorpion Venom. The 31st Congress on Science and Technology of Thailand, 18-20 Oct 2005, Suranaree University of Technology, Nakhon Ratchasima. การประชุมวิชาการวิทยาศาสตร์และเทคโนโลยีแห่งประเทศไทย ครั้งที่ 31 (วทท.31) ณ เทคโนธานี มหาวิทยาลัยเทคโนโลยีสุรนารี จ.นครราชสีมา 15. Sakda Daduang, Nunthawun Uawonggul, Arunrat Chaveerach, Sompong Thammasirirak, Tarinee Arkaravichien, Takeshi Nakamura, Michiyuki Matsuda, Michimoto Kobayashi, Seisuke Hattori, Manami Sone, Naoki Mochizuki. Purification and Characterization of Novel Toxin Heteroscorpine-1 (HS-1) from Heterometrus laoticus Scorpion Venom. The 2nd Yamada Symposium on Key Natural Organic Molecules in Biological Systems. 10-14 September 2005, Awaji Yumebutai International Conference Center, Hyogo, Japan 16. Nunthawun Uawonggul, Arunrat Chaveerach, Sompong Thammasirirak, Tarinee Arkaravichien, Wandee Bunyatratchata, Wipaporn Ruangjirachuporn, Pornpimol Jearranaiprepame, and Sakda Daduang. Investigation of Anti-microorganism Activity of Heteroscorpine-1 (HS-1), Neurotoxin from Heterometrus laoticus Thai Scorpion Venom. The 2nd Yamada Symposium on Key Natural Organic Molecules in Biological Systems. 10-14 September 2005, Awaji Yumebutai International Conference Center, Hyogo, Japan 17. Nunthawun Uawonggul, Sakda Daduang. Arunrat Chaveerach, Sompong Thammasirirak and Tarinee Arkaravichien. Screening of plants act against Heterometrus laoticus venom activity on fibroblast cell lysis. The 30th Congress on Science and Technology of Thailand, 19-21 Oct 2004, Impact Exhibition and Convention Center, Muang Thong Thani, Bangkok, Thailand. 18. Chidchanok Khamlert, Sompong Thammasirirak, Nunthawun Uawonggul, Thanawitch Lawitpithayawong, Mikio Kobayashi and Sakda Daduang, Development of Urease Strip for Heavy Metal Determination, The second Thailand Research Fund (TRF) Undergraduate Technology Development Exhibition, 30 April 2 May 2004 IT Mall, Fortune Town, Bangkok. Thailand. 19. Sakda Daduang, Nunthawun Uawonggul, Arunrat Chaveerach, Sompong Thammasirirak and Tarinee Arkaravichien, Production of antibodies against proteins in giant scorpion (Heterometrus laoticus) venom. The 29th Congress on Science and Technology of Thailand, 20-22 Oct 2003, Golden Jubilee Convention Hall, Khon Kaen University, Khon Kaen, Thailand 20. Daduang, S., Sattayasai, N., Sattayasai, J., and Chahomchuen, T. (2001) Localization of 42 kDa Glutamate-Sensitive Protein in Retina. Proc 19th International Australasian Winter Conference on Brain Research, 19-22 Aug 2001, Queenstown, New Zealand. 21. Daduang, S., and Fukui, Y.(1995) Studies of role of phosphatidyl inositol 3-kinase (PI3K) in cell contact inhibition. The 68th Japanese Biochemical Society Symposium, 16-18 Sep, Sendai, Japan (abstract in Japanese) 22. Daduang, S., Matsuda, M., Shizawa, Y., Fukui, Y. and Onodera, K. (1993) Production of monoclonal antibodies specific to carboxyl terminal Region of phosphatidylinositol 3-kinase (PI3K) 85 kD subunit. The 1993 Japan Society for Bioscience, Biotechnology, and Agrochemistry Symposium, 30 March3 April, Sendai, Japan (abstract in Japanese)
ได้รับทุนของ Japan Society for the Promotion of Science (JSPS) ระยะเวลา 1 สิงหาคม 2547 ถึง 31 มีนาคม 2548 ให้ไปทำงานวิจัย ณ Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita-shi, Osaka 565-0871, Japan