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ԭҵ
觷ع عǹ
ͻԭ ԷʵúѳԵ,ԭ
ҢԪ͡ ෤ժҾ / BIOTECHNOLOGY
ʶҺѹ֡ Է¢͹
稡֡һ .. 2532

ԭ
觷ع عǹ
ͻԭ ԷʵҺѳԵ,ԭ
ҢԪ͡ Ѫʵ / PHARMACY/PHARMACEUTICAL SCIENCE
ʶҺѹ֡ ԷԴ
稡֡һ .. 2534
ԷҹԾ Study of the Immunomodulating activity of hispidulin from Millingtionia hortensis Linn. F

ԭ͡
觷ع عѰ (ǧԷ : .. )
ͻԭ DOCTOR OF PHILOSOPHY
ҢԪ͡ Water Resources Planning and Management
ʶҺѹ֡ IOWA STATE UNIVERSITY
Ѱԡ
稡֡һ .. 2543
ԷҹԾ Study and Modeling of Atrazive Fate in Buffer strip Soils

çԨ

Phytoremediation of Carbofuran Residue in Soils
ع Research Center for Environmental and Hazardous Substance Management, Khon Kaen University
Ԩ 1. Mullika Teerakun
2. Alissara Reungsang
ҢԪ ෤ժҾ
ҢԨ ෤ժҾǴ
ʶҹҾ شç
Ѵ In this study, the ability of plants to clean up carbofuran, Furadan®, residue in rice field soil was examined. Three representative groups of plant species were studied i.e., grass crops (rice (Oryza sativa L.), cat tail (Typha angustifolia Linn.) and bulrush (Cyperus difformis Linn.)), upland crops (soybean (Glycine max Merr.), ground nut (Arachis hypogaea Linn.), mung bean (Vigna radiate (L.) Wilczek.), sunflower (Helianthus annuus Linn.) and corn (Zea mays L.)), and vegetable crops (water convolvulus (Ipomoea aquatica), tomato (Lycopersicon esculentum Mill), aubergin (Solanum aculeatissimum Jacq.), eggplant (Solanum melongena L.), chinese-kale (Brassica alboglabra Bailey.), chili (Capsicum annuum L.) and cabbage (Brassica oleracea var.capitata)). These plants were grown in 8 inches diameter plastic pot filled with soils containing 5 mg/kg carbofuran. Carbofuran residue in soil at day 0, 7, 14, 21, 35, 70, and 120 were sampled to analyze for concentration. The results showed that carbofuran was rapidly degraded under planted soil and non-planted soil with half-lives ranging from 2-7 days. These facts suggested that phytoremediation could accelerate the degradation of carbofuran residue in soil and carbofuran was not persistent in the soil environment. Grass crops and upland crops appeared to be suitable for phytoremediation of carbofuran residue in rice field soil because these plants could tolerate carbofuran and highly accumulated carbofuran in stems and leaves suggesting phytoaccumulation mechanism of these plants. Sunflower showed high amount of carbofuran, 93.4 g/kg dry weight, accumulated in its stems and leaves indicating a potential accumulator for phytoremediation of carbofuran.

Bioremediation treatment of MTBE and ETBE in contaminated soils
ع Research Center for Environmental and Hazardous Substance Management, Khon Kaen University
Ԩ 1. Pojanakorn Charathirakup
2. Alissara Reungsang
ҢԪ ෤ժҾ
ҢԨ ෤ժҾǴ
ʶҹҾ شç
Ѵ Three MTBE degraders were isolated from gasoline contaminated soil viz., KMS, KGS1 and KGS2. These isolates were tested for the ability to degrade ETBE at the concentration of 100 mg/L and to degrade a mixture of MTBE and ETBE in the NB media at the concentration of 50 mg/L each. The results showed that KGS1 was the best degraders in which 74% of MTBE alone, 25% of ETBE alone and 16% of MTBE and 23% of ETBE in the mixture were degraded, within 30 days. KGS1 was then further used in the experiments. Degradation of MTBE increased from 34% to 61% when KGS1 was amended in soil mixed with the combination of MTBE and ETBE. However, KGS1 did not significantly accelerate the ETBE degradation when it was amended in soil. One percent glucose significantly stimulated the degradation of MTBE by the indigenous microorganisms. The presence of KGS1 and an addition of 1% glucose as extra carbon source improved the degradation of MTBE, from 42 to 51%, suggesting KGS1 played an important role in the degradation of MTBE. KGS1 did not promote the degradation of ETBE alone but did on the MTBE and ETBE mixture. In conclusion, our isolated MTBE degrader-mixed culture was capable of degrading MTBE and ETBE. Bioaugmentation technique was more effective in remediating MTBE and ETBE in soil than biostimulation technique using 1% glucose.

Biohydrogen Production from Cassava Starch Manufacturing Wastewater
ع ӹѡҹͧعʹѺʹعԨ (ʡ.)
Ԩ 1. Alissara Reungsang
2. Suksaman Sangyoka
3. Tsuyoshi Imai
4. Pawinee Chaiprasert
ҢԪ ෤ժҾ
ҢԨ ժҾǴ (ѧҹ)
ʶҹҾ ҧԹ
Ѵ Batch production of biohydrogen from cassava wastewater were investigated using (i) anaerobic seed sludge, (ii) a mixed culture of anaerobic seed sludge and Rhodospirillum rubrum, and (iii) a two-step batch culture of anaerobic seed sludge and R. rubrum. Maximum hydrogen production in batch culture of anaerobic seed sludge was achieved at 55 oC and pH 5.0 with a specific hydrogen production of 429 mL H2 g-1-VSS and a hydrogen yield of 71.3 mL g-1-COD. Results from hydrogen production by the mixed culture showed that the presence of R. rubrum improved the specific hydrogen production by 1.5-fold and the hydrogen yield by 2.1-fold when compared to the use of anaerobic seed sludge at the same conditions of 30oC and pH 7.0. Superior results were obtained when the two-step batch culture, which involved the sequential addition of anaerobic seed sludge and R. rubrum, was used for hydrogen production. The cumulative hydrogen of 67 mL was produced at 30oC and pH 7.0. Our results suggested that cassava wastewater is one of potential sources of renewable biomass to produce hydrogen.

Quality indices of Nham, a traditional Northeastern Thai fermented pork sausage.
ع ٹԨ¡ѡŤҼԵѳҧɵ
Ԩ 1. On-Anong Chaiyachet
2. Alissara Reungsang
3. Kasem Nantachai
ҢԪ ෤ժҾ
ʶҹҾ شç
Ѵ Quality indices of Nham, 15 different brands, commercially produced in the Northeastern part of Thailand was formed using physico-chemical properties, microbiological properties and sensory evaluation. The analysis of samples indicated that their microbiological and chemical properties met the standard guidelines set by the Food Analysis Division, Ministry of Public Health and Thai Industrial Standard Institute. Statistical analysis between panelists acceptance and sensory properties of Nham suggested that flavor of Nham products were significantly affects on panelists acceptability (P < 0.05). Flavor of Nham as related with lactic acid (P < 0.05), content of lactic acid which higher panelists acceptance in flavor had approximated 0.54%. The results from overall acceptability and physical and microbiological properties analyzed of Nham samples showed that redness, hardness, adhesiveness and lactic acid bacteria effect on overall acceptability (P < 0.05). Characteristics of Nham had higher overall sensory panel score have pH, lactic acid content, lightness (L*), redness (a*), yellowness (b*), hardness and adhesiveness of Nham samples were 4.56, 0.54 % (w/w), 48.12 ± 1.26, 8.23 ± 1.41, 9.00 ± 0.91, 1.1734 ± 0.047 kg, 6.3532 ± 0.018 kg s, respectively. Mean lactic acid bacteria counts of Nham samples were about 5 × 107 CFU/g .

Isolation of Carbofuran Degraders from Phytoremediated Rhizosphere soil
ع National Research Center for Environmental and Hazardous Waste Management; Research Center for Environmental and Hazardous Substance Management
Ԩ 1. Pensri Plangklang
2. Alissara Reungsang
3. Wanpen Virojanakud
ҢԪ ෤ժҾ
ҢԨ ෤ժҾǴ
ʶҹҾ شç
Ѵ An enrichment technique was used to isolate carbofuran degraders from phytoremediated rhizosphere soil of rice (Oryza sativa L.), corn (Zea mays L.) and Chinese kale (Brassica alboglabra C.) used in phytoremediation study. Rhizosphere soil from each plants was added to C-, N- and C and N-limited BSM and complete media containing 5 mg/L of carbofuran as a sole C-, N- and C and N-source. The results indicated that carbofuran was dissipated in media. The percentage of dissipation of carbofuran in enriched media was in the range of 73-98%, 25-66%, 19-57% and 8-28% when the initial carbofuran concentrations were 5, 20, 40 and 100 mg/L, respectively. API 20 NE system was used to identify the gram negative-rod single isolates. They were identified as Burkholderia sp., Pseudomonas sp. and Stenotrophomonas maltophilia. These carbofuran degraders used carbofuran as a sole C-, N- or C and N- sources.

Effect of Carbon to Nitrogen ratio on the Composting of Cassava Pulp with Swine Manure
ع ӹѡҹͧعʹѺʹعԨ (ʡ.)
Ԩ 1. Nattipong Kamolmanit
2. Alissara Reungsang
ҢԪ ෤ժҾ
ҢԨ ෤ժҾǴ
ʶҹҾ ҧԹ
Ѵ This research investigated the effect of C/N ratio on the composting of cassava pulp with swine manure and to evaluate the physicochemical and biological properties of compost during the decomposition process. First set of experiment was set up consisting of cassava pulp with swine manure at an initial C/N ratio of 20:1 (pile 1), 30:1 (pile 2) and 40:1 without the seeding microorganism (p.d.1). Second set of experiment (pile 4, pile5 and pile 6) was set up similar to the first set but the seeding microorganisms (p.d.1) were added to each pile for accelerating the decomposition process. The moisture content in each pile was adjusted to 60 % before starting the composting process. The process was carried out in turned pile and the main process parameters were monitored for 84 days. The results indicated that a compost pile at an initial C/N ratio of 30:1 with an addition of p.d. 1 (pile 5) showed a faster rise in temperature, higher maximum temperature, longer thermophilic phase, lower faecal coliforms populations than the other piles. Chemical and biological parameters in pile 5 including total organic carbon (26.91%), total nitrogen (2.09%), total phosphorus (2.33%), total potassium (1.69%), C/N ratio (13:1), and pH (7.67) indicated that pile 5 achieved maturity after day 42. Therefore an effective ratio of C/N for composting of cassava pulp with swine manure was at 30:1 with an addition of p.d.1.

֡Ҥ㹡ҡС͹ԹҡкӺѴçҹصˡԵû
ع ѷԷԹѹ
Ԩ ͧʧ 觡ҧ ԩѵ ѡ آҹ ѧ¤
ҢԪ ෤ժҾ
ҢԨ ෤ժҾǴ
ʶҹҾ شç
Ѵ ҹԨ¹ըشʧ֡ǷҧªͧҡС͹ԹҡкӺѴصˡԵû·ӡüѹѵǹͧҳõչͧҡС͹Թ§ҷͧҴ 3 ѵǹ 0:1, 1:3 1:1 駹˹§ҷ 3 ѵǹ ջҳõչҡѺ 30 繵 ҡ鹹ٵ§ҹӨ״Դͻҹҵ¹ лҴء ӡêѴҴ鹡÷ͧҧ÷ͧء1ѻ֡ҷ4 ѻ Ѻҹ 10 ѻ Ѻҵ¹ 9 ѻѺҴء ҡ֡ҵҷ˹ѵҡԭԺ⵨ТͧЪԴ§§ҷѵǹõչͧҡС͹û0:1,1:31:1մѧҹѵҡԭԺ⵨ҡѺ 2.2, 2.2 2.0 繵ѹ ӴѺ ҵ¹ѵҡԭԺ⵨ҡѺ1.0, 1.5 1.1 繵ѹ ӴѺ лҴءѵҡԭԺ⵨ҡѺ 1.8, 2.4 1.8 繵ѹ ӴѺ ·ûѵǹѵҡԭԺ⵨㹻ЪԴդᵡҧѹҧʶԵ (p>0.01) ҡäӹdzѵҡ¹ͧ͢ЪԴ§§ѵǹ ѵҡ¹ͧ͢ҹŻҳ 17-19 ҵ¹ҳ 44-57 лҴءȻҳ 11-17 駹ѵҡ¹ٵèѴդҤ͹ҧ٧ͧҡѵդسҷҧҡ÷դèѵҡ¹͹¡ҡѺ 2 ҡһԷҾõչâͧҹӨ״Դ §÷ѵǹ һԷҾõչûҷѵǹ㹪ǧ 0.06-0.21 觨Ѵ繤ҷ¡Ҥҵðҹ 0.5 駹ѧࡵ÷§ҵͧҴһԷҾõչ¡Ҥҵðҹǡѹͤӹdzŵͺ᷹ҧɰʵ쾺ѵǹõչͧҡС͹ûҵͧҴѵǹѺҹлҵ¹1:1ҨЪ»Ѵ44.5繵ѵǹõչͧҡС͹ûҵͧҴѺҴءȤ 1:3Ъ»Ѵ20.5繵ҡسҾӡ͹ҧ§ ҫʹբͧӵ§ҷѺ÷ǹͧҡС͹դᵡҧҡ㹵§ҷѺûҵͧҴʴҡҡС͹ҡкӺѴ·ǹ§ҧ

֡зͧôٴѺͷ鹼
ع Research Center for Environmental and Hazardous Substance Management
Ԩ 1. ͧʧ
2. ɰ ԭش]
3. ķʹ
ҢԪ ෤ժҾ
ҢԨ ෤ժҾǴ
ʶҹҾ شç
Ѵ ÷ͧըشʧ֡Ƿҧ㹡ôٴѺͷ鹼 ·ӡ÷ͧз㹡ôٴѺ 繡ô-ҧͧ»ͷ ѵǹ鹼»ͷ 㹡ôٴѺ ҡ֡Ҿ зشͧôٴѺͷ鹼 ѵǹ鹼»ͷ 1:50 ·»ͷդ 繡ô-ҧ 8 ҷŤ 1 ͡ҡӡ֡ͧôٴѺ 觾ͧôٴѺѡɳ ¨Ѵ繪Դ (S-Type) Դ͵Ƕ١¶١ٴѺǵǴٴѺ ͧҡç֧ٴҧǶ١еǷդ٧ ͵Ƕ١¶١ٴѺŧ˹ҢͧǴٴѺ šŷШʶù¡šŷСѹ繡 ѧ鹶ͨҧաôٴѺ դҡ Ƕ١ٴѺСѹ繡 ٴѺҡ ͤӹdzҳôٴѺ٧ش BET Model ҤҡôٴѺ٧شҡѺ 1.00 x 10-4 ԡ.ͷ/.鹼 Ũҡ÷ͧ鹼ѡҾ㹡ù任ء㹡úӺѴ·軹͹»ͷ

FACTORS AFFECTING BIOHYDROGEN PRODUCTION FROM SQUEEZED JUICE OF PINEAPPLE WASTE BY Rhodospirillum rubrum UNDER NON-STIRRING CONTINUOUS ILLUMINATION FERMENTATION AND NON-STIRRING PERIODIC ILLUMINATION FERMENTATION
ع ͧع͹ѡѧҹ
Ԩ Piyawadee Ruknongsaeng and Alissara reungsang
ҢԪ ෤ժҾ
ҢԨ ෤ժҾǴ (ѧҹ)
ʶҹҾ شç
Ѵ This research examined the factors affecting biohydrogen production from squeezed juice pineapple waste in batch culture by photosynthetic bacteria strain Rhodospirillum rubrum. The fermentation conditions used in this study were non-stirring continuous illumination fermentation and non-stirring periodic illumination fermentation. Acetic acid and propionic acid were used as carbon sources with the initial concentrations ranged from 5-20 mM. Ammonium sulfate at the initial concentration of 2 and 10 mM was used as a nitrogen source. The total volume and the compositions of evolved gas over time were determined and then fitted to the modified Gompertz equation. The results showed that non-stirring periodic illumination fermentation was significantly effected on biohydrogen production (P ≤ 0.05). The maximum accumulative hydrogen and the maximum hydrogen production rate were 337 ml and 12.8 ml/h, respectively.

Factors affecting hydrogen production from cassava starch wastewater by a combination of anaerobic mixed culture and photosynthetic bacteria
ع ӹѡҹͧعʹѺʹعԨ (ʡ.)
Ԩ 1. Suksaman Sangyoka
2. Alissara Reungsang
3.Tsuyoshi Imai
ҢԪ ෤ժҾ
ҢԨ ෤ժҾǴ (ѧҹ)
ʶҹҾ ҧԹԨ
Ѵ Batch experiments were conducted to investigate the effect of pH, nitrogen sources, reducing agents, mixing and light conditions on hydrogen production from cassava starch wastewater by a mixed culture of anaerobic seed sludge and photosynthetic bacteria (Rhodospirillum rubrum). Anaerobic seed sludge was used to produce organic acids as the intermediates which then converted to hydrogen by R. rubrum. The maximum specific hydrogen production of 390 ml/g-VSS-d with a specific hydrogen production of 2,064 mL H2 g-1-VSS and a hydrogen yield of 343 mL g-1-COD were obtained at an initial pH of 7 and ammonium phosphate, Enterobacter aeragenese were used as N-source and reducing agent, respectively under a mixing and non-continuous light conditions. The biogas at these conditions contained up to 50-60% of hydrogen.

ŧҹԨ/Ԫҡ
  • Charathirakup P., Reungsang A. and Wirojanagud W. 2004. Comparision of an Abillity to Degrade MTBE between Mixed Culture and Monoculture Isolated from Gasoline Contaminated Soil. Songklanakarin Journal of Science and Technology. 26(1), 109-116. (Correspondence Author)
  • eerakun M., Reungsang A. and Wirojanagud W. 2004. Phytoremediation of Carbofuran Resdues in Soil. Songklanakarin Journal of Science and Technology. 26(1), 171-176. (Correspondence Author)
  • Rittirod T, Tattawasart A, Kumkainam K, Setsomboon W, Thongchai J, Tessiri T, Reungsang A. and Skolchai S. 2003. Efficacy of a Washing Machine for Small Volume Glass Vial Used in Phamaceutical Analysis. KKU Engineering Journal. 30(4), 287-293.
  • Reungsang, A., T.B. Moorman, and R.S. Kanwar. 2001. Transport and Fate of Atrazine in
    Midwestern Riparian Buffer Strips. JAWRA. 37:1681-1692.
  • Moorman, T.B., K. Jayachandran and A. Reungsang. 2001. Adsorption and Desorption of
    Atrazine in Soils and Subsurface Sediments. Soil Science. 166:921-929.
  • Reungsang, A. 2001. Effect of Swine Manure Application on Atrazine Mineralization, Biomass
    Carbon and Atrazine Degradation. KKU Engineering J. 28: 115-126.
  • Reungsang, A. 2001. Dissipation of Rice Pesticides in Water. KKU Engineering J. 28:49-58.
  • Reungsang, A., T.B. Moorman, and R.S. Kanwar. 1997. Effect of Swine Manure Application on Atrazine Mineralization, Biomass Carbon and Atrazine Degradation. ASAE Paper No. 972150. International Meeting of the American Society of Agricultural Engineering, Minneapolis, MN.
  • Reungsang, A., R.S. Kanwar, and T.B. Moorman. 1996. Effect of Soil Depth and Swine Manure on Sorption and mineralization of Atrazine. ASAE Paper No. MC96-130. Mid-Central ASAE Meeting, St. Joseph, MO.
  • Teerakun M., Reungsang A. and Wirojanagud W. 2005. Determination of Plant Species for the Phytoremediation of Carbofuran Residue in Rice field Soil. The 4th National Environmental Conference. 19-21 January, Chonburi, Thailand. pp. 246-254.
  • Plangklang P., Reungsang A. and Wirojanagud W. 2005. Isolation of Carbofuran Degraders from Phytoremediated Soil. The 4th National Environmental Conference. 19-21 January, Chonburi, Thailand. pp. 342-350.
  • Charathirakup P. and Reungsang A. 2005. Bioaugmentation and Biostimulation of MTBE Degrader for Degrading MTBE and ETBE. The 4th National Environmental Conference. 19-21 January, Chonburi, Thailand. pp. 795-803.
  • Sangyoka S., Reungsang A. and Imai T. 2005. Factors Affecting Hydrogen Production from Cassava Starch Wastewater by a Combination of Anaerobic Mixed Culture and Photosynthetic Bacteria. The 1st International Conference on Fermentation Technology for Value Added Agricultural Products. 22-25 March, Khon Kaen, Thailand.
  • Kamolmanit N. and Reungsang A. 2005. Effect of Carbon to Nitrogen Ratio on the Composting of Cassava Pulp with Swine Manure. The 1st International Conference on Fermentation Technology for Value Added Agricultural Products. 22-25 March, Khon Kaen, Thailand.
  • Reungsang A., Sangyoka S., Imai T. and Chaiprasert P. 2004. Biohydrogen Production from Cassava Strach Manufacturing Wastewater. Proceeding of JGSSE and Kyoto University Joint International Conference on Sustainable Energy and Environment. 1-3 December 2004. Itilton Hua Hin Resort & Spa, Hua Hin, Thailand. Vol. 1, p 319-324
  • Reungsang, A., Prasomsat, P., Plangklang, P. and Phesatcha, P. 2003. The Study on the
    Possibility of Using the Activated Sludge from the Wastewater Treatment of Wunsen
    Industry for Fish Food.The 15th National Confference of the Environmental Engineering
    Association of Thailand. BITEC Convention Center. July 24-25, 2003. 74-80.
  • Reungsang, A. and Sangyoka, S. 2003. Carbofuran Persistence in Saline Soil and Saline
    Water. Proceeding of the International Conference on Water Resources Management for Safe Drinking Water. Novotel Hotel, Chiang Mai, Thailand. March 25-29, 2003. 23-29.

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